NALF is an abbreviation for "Nucleic Acid Lateral-Flow", which describes technologies detecting molecular amplification products on a lateral-flow strip. Typically NALF systems combine lateral-flow strips with a microfluidic system.
The method used by AMODIA has the following specifications:
- the amplification product is labeled during amplification
- to this labeled amplification product a labeled probe is hybridized (sequence verification !)
- one label binds to the particles on the lateral-flow strip
- the other label binds to capture molecules immobilized on the membrane of the LFD
- all target molecules are amplified in parallel with an internal control, which binds according to the same principle on a separate line
- the proper functioning is displayed by an additional line (assay control)
AMODIA offers several variations of lateral-flow strips (e.g. the MultiFlow® Basic), which differ in the number of amplification products detectable in parallel.
Within a research project (PTJ: 0315656A) AMODIA was able to demonstrate the combination of lateral-flow strip and microfluidics on a chip.