The Universal Molecular Identification allows to detect microorganisms present in a sample without any knowledge about which and how many different microorganisms to expect. The detection is independent of culturing, so it displays the direct semi-quantitative state of the microbial community at the time of sample collection.
This approach has many advantages:
- all microorganisms in the sample may be identified
- the identification is performed without foreknowledge about the type of microorganisms
- no distraction or influence of the microbial community by the type of culturing
- unknown contaminating microorganisms may be classified
AMODIA offers several different variations of universal and group-specific analysis which differ in the respective target genes:
| Available Target Genes for: | Bacteria | Fungi |
|---|---|---|
| Universal (bold: standard system) |
16S rRNA V2-V3 16S rRNA V4-V5 16S rRNA V8-V9 rpoB Silva system* |
18S rRNA 28S rRNA ITS |
| Group of Methanogens | 16S rRNA mcrA |
|
| Group of Sulfate Reducers | dsrAB | |
| Group of Sulfur Oxidizers | soxB |
*: Klindworth et al. (Nucleic Acid Research, 2013, Vol. 41, No 1 e1)
Two application examples for the Universal Molecular Identification are presented here with the analysis of a washing machine and groups of bacteria, respectively. Possible other areas of application are:
- environmental samples, e.g. waterbody samples, drinking water, soil samples, sediments, biofilms
- samples from geological measurement stations
- biogas plants or facilities for the fermentation of mixed populations
- damages by microbial corrosion
- industrial filling or coating facilities
- quality control of equipment, raw materials or products
- input of contaminations into facilities or products
- verification of biocides or hygiene measures
- moulds on wallpapers, wood or in silicone joints
- medical sample material with mixed infections
- and many more
The technique allows to identify microbial contaminations subsequently. It can be used as origin for developing an on-site detection system specific for those contaminants.
On request AMODIA develops group-specific systems according to customer-specific requirements. Please inquire for more information!
Additional information may be found here: