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Strain typing of isolates allows to compare and differentiate strains of the same species. For this purpose we use specific PCR techniques.

Typical applications for strain typing are:

 

Depending on the microorganism different methods are used for strain typing:


In addition to the test isolate(s) a defined reference isolate is required. The PCR products are separated on acrylamid gels (v. fig.). The band pattern is analysed with a specific program for densitometric analyses. Additionally for several isolates a phylogenetic tree may be generated to visualize the genetic similarities.

Additional information may be found here: