Technologies
Technique of Universal Molecular Identifications
This technique is based on so-called "phylogenetic markers". Examples for such markers are the 16S rRNA gene for bacteria or the 18S rRNA for fungi, respectively.
The DNA of all microorganisms present in the sample is amplified using universal primers. Amplification products are then separated according to their sequence. This "screening" step displays a gel picture, where every sample is represented by a lane, and every microorganisms by a band.
In a second step the DNA in each band can be sequenced. Finally these sequences are compared with sequence data of known microorganisms, thus identifying the next-homologous sequence and species.
AMODIA offers the following universal and group-specific systems:
- universal identification of bacteria by 16S rRNA
- universal identification of sulfate-reducing bacteria (SRB)
- identification within the group of methanogenic bacteria
- universal identification of yeast and fungi by 18S rRNA (combination)
- universal identification of eucaryotes using the ITS region
Lateral-Flow Dipstick (LFD)
A lateral-flow dipstick (short: LFD) is a detection platform based on the lateral-flow principle. This is capillary forces move a liquid along the surface (= lateral) of a membrane.
Neither use nor read-out of a LFDs require a device: the liquid transport is done by capillary forces and the read-out is possible by the human eye. Expensive detection technique is not required.
Nucleic Acid Lateral-Flow (NALF)
NALF is an abbreviation for "Nucleic Acid Lateral-Flow", which describes technologies detecting molecular amplification products on a lateral-flow strip.
AMODIA offers several variations of lateral-flow strips, which differ in the number of amplification products detectable in parallel.